Welcome to Canine K9 Progesterone Step by Step Instructions
Step 1
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Step 1
To begin using a Canine K9 Progesterone testing product, first ensure that all required supplies are prepared, including the progesterone test kit, sterile needles and syringes, blood collection tubes, pipettes, gloves, and any analyzer or centrifuge required by the product. Always confirm that the test kit is within its expiration date and stored according to the manufacturer’s guidelines.
Step 2
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Step 1
Next, collect a blood sample from the dog while she is calm and properly restrained.
Blood is typically drawn from a leg or neck vein using sterile equipment.
Most progesterone tests require approximately one milliliter of blood, although the exact amount depends on the specific kit being used.
Step 3
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Step 3
After the blood sample is collected, allow it to clot if the test requires serum. This usually takes about fifteen minutes at room temperature. Once clotted, place the sample into a centrifuge to separate the serum from the blood cells.
Carefully remove the clear serum for testing, avoiding samples that appear red or cloudy, as this may affect accuracy. If the test kit allows the use of whole blood, proceed according to the manufacturer’s instructions without centrifugation.
Step 4
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Once the sample is prepared, use a pipette to transfer the required amount of serum or whole blood into the test cassette or sample well. Follow the kit’s instructions closely regarding sample volume, as adding too much or too little can impact the result.
Step 5
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After loading the sample, allow the test to incubate for the specified amount of time, typically around fifteen minutes. During this period, keep the test at a stable temperature and avoid moving or disturbing it.
Step 6
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When incubation is complete, insert the test cassette into the analyzer if one is used, or read the visual results according to the test guide. Most tests provide progesterone levels in nanograms per milliliter (ng/mL), which indicate the dog’s reproductive stage.
About Canine K9 Progesterone
Step 7
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Interpret the progesterone results by comparing the numeric value to standard breeding ranges. Lower values usually indicate early heat, rising values suggest ovulation is approaching, and higher values confirm that ovulation has occurred. Because progesterone levels change rapidly, testing should be repeated every one to three days to accurately track hormonal progression.
Step 8
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Finally, record each test result along with the date and day of the heat cycle. Maintaining accurate records helps identify the optimal breeding window and supports consistent reproductive planning.
